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Chromatography separates mixture components by exploiting different affinities/partitioning between a mobile phase and a stationary phase.
Chromatography is a laboratory technique used to separate a mixture into its individual components. The sample is carried by a mobile phase (a gas or liquid) through a system such as a column, capillary, plate, or sheet, where a stationary phase is fixed. Different constituents interact differently with the stationary phase, so they are retained for different lengths of time and therefore move through the mobile phase at different apparent velocities, leading to separation based on differential partitioning between the two phases.
Chromatography separates mixture components by exploiting different affinities/partitioning between a mobile phase and a stationary phase.
Components travel at different rates because of different retention on the stationary phase, producing separated outputs (e.g., bands or peaks).
Chromatography can be used for purification (preparative) or for identifying and measuring analytes in smaller samples (analytical).
A physical separation method that distributes components between a stationary phase and a mobile phase to achieve separation.
The phase (gas or liquid) that moves in a definite direction and carries the sample through the chromatographic system.
The material fixed in place during chromatography that interacts with sample components and causes differential retention.
The substance(s) in the sample that are targeted for separation and/or measurement during chromatography.
Chromatography performed to purify enough of a substance for later use rather than just analysis.
Chromatography performed to determine the presence and/or relative proportions (concentrations) of analytes in a sample.
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